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Catalog# Product Description |
ICP1325 This antibody was developed using Tthyroxine (T4) linked to the immunogenic carrier protein -KLH conjugates as the immunogen, The T4-specific antibodies was and affinity purified with antigen-specific affinity chromatography and eluted by competitive free T4. The antibody could be utilized for detection, quantization and affinity isolation and purification of estradiol in biofluid. |
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Competitive ELISA
  Structure of the T4 Immunogen
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Species
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Rabbit
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Formulation
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250 μg/mL in PBS with 50% glycerol
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Immunogen
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T4-linked KLH protein conjugates
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Purification
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Antigen-specific immune-affinity chromatography and free T4 competitive elution
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Specificity
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The antibody recognizes free estradiol.
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Applications
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Quantitative ELISA: IC50=0.8 ng/mL, Immunoaffinity Chromatography
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Scientific Description
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Thyroxine, also called 3,5,3′,5′-tetraiodothyronine, or T4, one of the two major hormones secreted by thethyroid gland (the other is triiodothyronine). Thyroxine’s principal function is to stimulate the consumption of oxygen and thus the metabolism of all cells and tissues in the body. Thyroxine is formed by the molecular addition of iodine to the amino acid tyrosine while the latter is bound to the protein thyroglobulin. Excessive secretion of thyroxine in the body is known as hyperthyroidism, and the deficient secretion of it is called hypothyroidism
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is one year from date of shipping if properly stored.
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