掃一掃，添加微信號
|
Catalog # Product Description |
ICP0851 This high affinity His-tag antibody HRP conjugate is developed using a polyhistidine peptide (HHHHHH) conjugated to KLH and affinity-purified with the HHHHHH peptide. The antibody is directly conjugated to peroxidase (HRP) and is useful for detection and localization of His-tag proteins. |
|
Western-blot with His-tag antibody, HRP (ICP0851) of the His-tag protein (50 kDa) expressed cell lysate and enriched His-tag protein by IP with anti-His-tag (ICP0850) 1: Molecular marker 2: 10 μL of His-tag protein in lysate (original cell lysate) 3: 10 μL of His-tag protein in lysate (after IP) 4: 10 μL of the His-tag protein in 100 μL of the IP eluting buffer, enriched with 25 μg of the anti-His tag (ICP0850) from 2 mL of the original lysate
A: 5 μg of His-tag fusion protein (50 kDa) B: 20 μg His-tag fusion protein (50 kDa)
|
|
Species
|
Rabbit
|
||
|
Formulation
|
250 μg/mL in PBS, 50% glycerol
|
||
|
Immunogen
|
Polyhistidine peptide (HHHHHH) KLH conjugates
|
||
|
Purification
|
Antigen-specific affinity chromatography
|
||
|
Specificity
|
The antibody recognizes His-tag proteins.
|
||
|
Applications
|
ELISA (1:2500); WB (0.25-1.0 μg/mL); IHC (5 μg/mL)
|
||
|
Scientific Description
|
Polyhistidine peptide is frequently used as an affinity tag to facilitate the isolation of the 6H-fusion proteins using a metal-chelate matrix.
|
||
|
Storage & Stability
|
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
|
