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Catalog # Product Description |
CN0885 This high affinity anti-GST-tag antibody is developed using the GST peptide conjugated to KLH. The peptide sequence (EEHLYERDEGDKWR) is derived from the common vector glutathione S transferase (protein code: ACH81576). |
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Western blot analysis of cell expressing a GST fusion protein (45 kDa). Left lane: 5 μL of the negative cell lysates; right lane: 5 μL of the cell lysate, transfected with a vector coding GST-fusion protein.
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Species
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Rabbit
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Formulation
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250 μg/mL in PBS with 50% glycerol
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Immunogen
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GST peptide EEHLYERDEGDKWR KLH conjugates
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Purification
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Antigen-specific affinity chromatography
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Specificity
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The antibody recognizes the 37 kDa GADPH protein as a single band.
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Applications
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ELISA (1:2500); WB (1:500-2000); IF (5 μg/mL); IHC (5 μg/mL)
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Scientific Description
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GST is glutathione S transferase which is frequently used as an affinity tag in recombinant proteins to facilitate the isolation and localization of the target protein.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is three years from date of shipping if properly stored.
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