掃一掃，添加微信號
Phosphotyrosine Antibody, HRP
|
Catalog# Product Description |
ICP9902 The affinity purified rabbit polyclonal anti-phospho-tyrosine antibody was conjugated to horseradish peroxidase (HRP). The conjugates are useful for detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides. |
|
Immunoprecipitated samples with anti-phospho-tyrosine agarose (ICP9804)
Left: Mouse spleen lysate treated with complete adjuvant Right: Mouse spleen lysate without any treatment Detection: Phospho-tyrosine antibody, HRP (ICP9902)
|
|
Species
|
Rabbit
|
||
|
Formulation
|
250 μg/mL in HRP stabilizing buffer with 50% glycerol
|
||
|
Immunogen
|
Iodoacetylphosphotyramine-KLH conjugates
|
||
|
Purification
|
The antibody was affinity purified with phospho-tyrosine immobilized on agarose, followed by immune affinity absorption with tyrosine on agarose.
|
||
|
Conjugation
|
Reductive amination; HRP/Ab ratio - 10:1
|
||
|
Specificity
|
The antibody is pan-specific against phospho-tyrosine. There are no cross reactions with phospho-serine or phospho-threonine.
|
||
|
Applications
|
ELISA (1:1000); WB (1:1000)
|
||
|
Scientific Description
|
Tyrosine is one of the 20 naturally occurring amino acids used for the biosynthesis of proteins. Kinases phosphorylate tyrosine to form phospho-tyrosine which is considered a key step in the regulation of enzymatic activities such as signal transduction.
|
||
|
Storage & Stability
|
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is six months from date of shipping if stored properly.
|
||
|
Product Specific References |
|
