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Catalog#
Product Description
ICP9904
The affinity purified rabbit polyclonal anti-phospho-serine (anti-pS) was developed using phospho-serine-KLH conjugates and a phospho-protein mixture as the immunogen. The phospho-serine specific antibody was affinity purified with serine-phosphorylated peptide immobilized to agarose. The affinity-purified anti-pS was conjugated to peroxidase (HRP) by reductive amination. Direct application of the primary conjugates will avoid non-specific signals, particularly due to the antibody signal on Western blot analysis of IP's with antibodies from the same species.
Product Specific References
Species
Rabbit
Formulation
250 μg/mL in PBS, 50% glycerol
Immunogen
Phospho-serine-KLH and phosvitin mixture
Purification
The antibody was purified with phospho-serine on agarose.
Conjugation
Reductive amination; HRP/Ab molar ratio - 1:10
Specificity
The antibody recognizes proteins phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine.
Applications
ELISA (0.25 - 1.0 μg/mL); WB (0.25 - 1.0 μg/mL)
Scientific Description
Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to form phospho-serine, which is responsible for various signalling processes in eukaryotes. [1]
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is six months from date of shipping if properly stored.
