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Catalog# Product Description |
ICP0115 S6 peptide (KRRRLASLR) is a substrate of S6K and ILK. Affinity purified rabbit polyclonal anti-pS6 antibodies were developed with the phosphorylated S6 (KRRRLApSLR) and absorbed with the non-phosphorylated S6 peptide. This antibody is conjugated to horse-radish peroxidase (HRP) through reductive amination. This antibody has been used for non-radioactive p70 S6K kinase assays. |
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Species
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Rabbit
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Formulation
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250 μg/mL in HRP stabilizing buffer, 50% glycerol
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Immunogen
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Phosphorylated S6 peptide KRRRLApSLR-KLH conjugates
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Purification
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Affinity purified with the phospho-peptide KRRRLApSLR on agarose and affinity absorbed with the non-phosphorylated S6 peptide
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Conjugation
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Reductive amination with 2/1 molar ratio of HRP/antibody
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Specificity
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The antibody recognizes only the phosphorylated product of S6K. There are no cross reactions with the non-phosphorylated peptide substrate.
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Tested
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Human breast cancer tissues and mouse cells
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Applications
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ELISA (0.25 - 1.0 μg/mL); WB (0.25 - 1.0 μg/mL); Non-radioactive S6K kinase assay
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Scientific Description
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The S6 substrate peptide (KRRRLASLR) is a substrate for p70 S6K and other kinases such as PKC, PKM and PKA.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is six months from date of shipping if properly stored.
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Product Specific References
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1. Pept. Res. 1991. 4(3): 158-170.
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