掃一掃，添加微信號(hào)
|
Catalog# Product Description |
ICP0180 Crebtide (KRREILSRRPSYR) is a substrate peptide derived from the protein Creb which is widely utilized as a synthetic substrate for the PKA and PKC kinase families. Affinity purified rabbit polyclonal anti-pCrebtide is developed with phosphorylated Crebtide (KRREILSRRPpSYR) and absorbed with non-phosphorylated Crebtide. |
|
Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay
Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay: A: 0.0 ng B: 3 ng C: 6 ng D: 12.5 ng E: 25 ng F: 50 ng
Western-blot analysis of the BSA-Crebtide phosphorylated with PKC alpha Kinase/assay: A:0.0 ng B:4 ng C:15 ng D:60 ng E:250 ng.
|
|
Species
|
Rabbit
|
||
|
Formulation
|
250 μg/mL in TBS, 0.1% NaN3
|
||
|
Immunogen
|
Phospho-Crebtide (KRREILSRRPpSYR)-KLH
|
||
|
Purification
|
The antibody was affinity purified with KRREILSRRPpSYR on agarose followed by affinity desorption with non-phosphorylated Crebtide.
|
||
|
Specificity
|
The antibody recognizes only the phosphorylated Crebtide product of PKA and PKC in a non-radioactive kinase assay. There are no cross-reactions with the non-phosphorylated Crebtide.
|
||
|
Applications
|
ELISA; WB; Non-radioactive PKA and PKC kinase assay
|
||
|
Scientific Description
|
p33 (ING1) is a new growth inhibitor candidate for a tumour suppressor gene. p33 (ING1) cooperates with p53 and down-regulates cell growth by activating transcription from the p21/WAF1 promoter although there is no sequence similarity between p33 (ING1) and p53.
|
||
|
Storage & Stability
|
Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at -20°C. Avoid cycles of freezing and thawing. Expiration date is one year from date of shipping if stored properly.
|
||
|
Product Specific References
|
