掃一掃，添加微信號
|
Catalog# Product Description |
ICP0506 Affinity purified rabbit polyclonal anti-methylated lysine antibody is developed using a methylated protein as the immunogen. The methyllysine specific antibodies are affinity purified with N-e-mono-methyllysine and di-methyllysine on agarose as the affinity matrix. The purified antibody was conjugated to fluorescein. The conjugated antibody was tested to localize methylated proteins. |
|
Species
|
Rabbit
|
|
Formulation
|
250 μg/mL in PBS, 50% glycerol
|
|
Immunogen
|
Methylated KLH conjugates
|
|
Purification
|
N-e-mono-methyllysine and di-methyllysine on agarose
|
|
Specificity
|
Recognizes proteins methylated on lysine residues (mono and di-methyllysine). It does not cross react with acetylated proteins. The cross reactivity to tri-methyllysine has not been tested.
|
|
Conjugation
|
With fluorescein isothiocyanate (FITC)
FITC/Ab molar ratio - 10:1
|
|
Applications
|
IF (1:50 - 1:100)
|
|
Storage & Stability
|
Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is six months from date of shipping if properly stored.
|
