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Methyl (mono) Arginine Antibody
Catalog#
Product Description
ICP0801
This antibody is raised from rabbits that were immunized with NG- mono-methyl arginine-KLH conjugates and was specifically isolated and affinity purified using the monomethyl-arginine antigen. The affinity purified methyl arginine antibodies are useful for detection, isolation and localization of proteins with Nγ-methylated arginine residues.
Competitive ELISA: inhibition of the binding mMeR-HRP to anti-mMeR antibody immobilized (1μg/mL) by free mMeR (IC50=40-50 ng/mL)
Fluorescent Staining of MMRU cells
The antibody recognizes free mono-methyl arginine and proteins with methylated arginine residues. It does not cross react with either asymmetric or symmetric dimethyl arginine.
Product Specific References
Western blot of human MMRU cells with ICP0801 (A,B) plus competitive inhibition with free methyl arginine (C,D)
Species
Rabbit
Formulation
250 μg/mL in PBS, 50% glycerol
Immunogen
NG-mono-methyl arginine-KLH conjugates
Purification
Purified by antigen-specific affinity chromatography
Specificity
Species Tested
Pan-specific regardless of species.
Applications
ELISA (1:2000); WB (1:1000); IP (5 μg/mg protein sample); IF (5 μg/mL); IHC (5 μg/mL)
Scientific Description
Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
Storage & Stability
Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is three years from date of shipping if properly stored.
