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Catalog# Product Description |
ICP9947 This antibody was developed using ochratoxin A (OA)-KLH conjugates, linked via its free carboxyl group. The antibody was affinity purified with an OA-agarose affinity column. The purified antibody was chemically conjugated to HRP. It could be utilized for direct competitive detection and quantization of the food-borne mycotoxin, ochratoxin A. |
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Direct competitive inhibition ELISA using immobilized anti-OA (1.5 μg/mL), and OA-labelled HRP (50 ng/mL). 50% inhibition occurred at 25 ng/mL of free ochratoxin A in non-optimized conditions.
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Species
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Rabbit
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Formulation
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0.25 mg/mL in stabilizing buffer
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Immunogen
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Pure ochratoxin A linked to KLH, via the free carboxyl group of OA using the NHS-active ester method.
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Purification
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Antigen-specific affinity chromatography
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Specificity
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The antibody recognizes ochratoxin A (100%), C (400%), B (5%), and <1% of ochratoxin alpha.
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Applications
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ELISA, affinity column enrichment
Note: For better ELISA results, use a different method than the NHS-active ester method to prepare the paired antigen, such as the OA-OV with EDC method.
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Scientific Description
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Ochratoxin A is a food borne mycotoxin which is carcinogenic and nephropathic to both humans and animals. Contamination with OA in food may be harmful to humans and animals.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date issix months from date of shipping if stored properly.
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Product Specific References |
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