Catalog#

Product Description

ICP1205

This antibody was developed using aflatoxin B1 (AFB1) BSA conjugates and affinity purified with an AFB1-Agarose affinity column and competitively eluted by free AFB1. The antibody could be utilized for detection and quantization of the food-borne mycotoxin AFB1.

Indirect competitive inhibition ELISA using immobilized AFB1-BSA (ICP0.1 μg/μL) and anti-AFB1 (0.1 μg/mL), 50% inhibition occurred at 0.7 ng/mL of free AFB1 in a non-optimized conditions.

Species

Rabbit

Formulation

250μg/mL in PBS with 50% glycerol or lyophilized powder

Immunogen

Pure AFB1 conjugated to BSA.

Purification

Antigen-specific affinity chromatography and

free antigen competitive elution system.

Specificity

The antibody recognizes aflatoxin B1 specifically; cross reactivity to other aflatoxins not tested yet.

Applications

ELISA, gold colloid strip test, IAC (affinity chromatography).

Scientific Description

Aflatoxin B1 is a food borne mycotoxin which is carcinogenic to both humans and animals. Contamination with AFB1 in food may be harmful to humans and animals.

Storage &

Stability

Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is one year from date of shipping if properly stored.