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Catalog# Product Description |
ICP9705 Monoclonal antibody production using cell culture systems sometimes requires the use of fetal calf serum. Purification of the mouse antibody from the cell culture supernatant using Protein A/G systems may result in contamination with bovine IgG. Anti-mouse IgG agarose is the mouse IgG-specific affinity matrix that could selectively purify mouse IgG from mouse serum, both ascites and the supernatant of the cell culture media, without pulling-down bovine IgG. This anti-mouse IgG agarose is prepared using antigen-specific affinity purified anti-mouse IgG as the immobilized antibodies. More than 20 mg of pure anti-mouse IgG was covalently immobilized onto the agarose beads via a highly stable thiol-ether bond. This product has been successfully used to replace Protein A/G agarose for more specific affinity purification of mouse IgG with high binding capacity (> 10 mg/mL). |
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Formulation
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1 mL beads in 2 mL 50% glycerol
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| Binding Capacity | >10 mg/mL of mouse IgG | |
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Applications
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Affinity chromatography ; Immunoprecipitation
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| Ligand Immobilized | 40 mg anti-mouse IgG/mL | |
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly.
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| Product Specific References | ||
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Purification of mouse IgG from monoclonal cell culture supernatant with anti-mouse IgG agarose.
Fraction 1: 0.8 mg/mL
Fraction 2: 2.5 mg/mL
IgG Std: 0.31 - 5 mg/mL
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